Experimental studies in pigs on Trichinella detection in different diagnostic matrices

Nöckler, K.; Serrano, F. J.; Boireau, P.; Kapel, C. M. O.; Pozio, E.

doi:10.1016/j.vetpar.2005.05.033

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Experimental studies in pigs on Trichinella detection in different diagnostic matrices

Nöckler, K.; Serrano, F. J.; Boireau, P.; Kapel, C. M. O.; Pozio, E.

A total of 72 specific pathogen-free (SPF) and Iberian pigs (three animals per group) were inoculated with 200, 1000 or 20,000 muscle larvae of T. spiralis, T. nativa, T. britovi and T. pseudospiralis. For each animal, the muscle larva burden was evaluated in nine muscle samples by digestion. The anti-Trichinella IgG kinetics in blood samples, taken twice prior and at days 5, 10, 15, 20, 25, 30, 40, 50 and 60 post-inoculation, and in muscle juice, obtained at necropsy, was evaluated by an ELISA using an excretory/secretory antigen. The mean larval recovery rate in SPF/Iberian pigs corresponded with the level of inoculum dose, and tongue, diaphragm and masseter were identified as predilection muscles. In SPF and Iberian pigs receiving 20,000 larvae of T. spiralis, an earlier seroconversion was detected from day 25 post-inoculation. At a 10-fold dilution, the muscle juice showed a good test agreement with blood serum

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Erschienen in:: Veterinary parasitology
Vol. 132, H. 1-2 (2005), S. 85-90
Band:: 132
Heft:: 1-2
Datum der Veröffentlichung:: 2005
DOI:: 10.1016/j.vetpar.2005.05.033
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: animal; Animals; Antibodies; Antibodies,Helminth; ANTIBODY; ANTIGEN; ANTIGENS; Antigens,Helminth; article; assessment; Berlin; BfR; blood; BRITOVI; chemistry; comparative; Comparative Study; detection; diagnosis; diagnostic; Digestion; ELISA; Enzyme-Linked Immunosorbent Assay; Germany; growth & development; Helminth Proteins; kinetics; Larva; LEVEL; methods; MUSCLE; muscle larvae; Muscle,Skeletal; NATIVA; parasitology; pig; PIGS; protein; Proteins; RECOVERY; Research; Risk; Risk Assessment; RISK-ASSESSMENT; SAMPLES; SERA; serum; Specific Pathogen-Free Organisms; SPIRALIS; study; Swine; Swine Diseases; Trichinella; Trichinella detection; Trichinella spiralis; TRICHINOSIS; veterinary
DDC-Sachgruppe der DNB:: 600 Technik
Link URL:: http://www.sciencedirect.com/science/article/pii/S0304401705002633
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)