Isolation and molecular characterisation of equine rotaviruses from Germany

Elschner, Mandy C.; Schrader, C.; Hotzel, Helmut; Prudlo, Jutta; Sachse, Konrad; Eichhorn, W.; Herbst, W.; Otto, Peter

doi:10.1016/j.vetmic.2004.10.010

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Isolation and molecular characterisation of equine rotaviruses from Germany

Elschner, Mandy C.; Schrader, C.; Hotzel, Helmut ; Prudlo, Jutta ; Sachse, Konrad ; Eichhorn, W.; Herbst, W.; Otto, Peter

A total of 26 rotavirus positive faecal samples of diarrhoeal foals, and 8 equine rotavirus isolates were examined. Viral RNA patterns were generated, G typing was performed by PCR, and a P[12]-specific DNA probe was developed for P typing. Furthermore, five equine rotavirus isolates were sequenced in the genomic regions coding for VP7 and part of VP4. Rotaviruses of genotype G3 P[12] were found in 22 faecal samples and G14 P[12] type could be found in 4 faecal samples. These findings confirm that in Germany G3 P[12] is the predominating type of equine rotaviruses. (C) 2004 Elsevier B.V. All rights reserved

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Erschienen in:: Veterinary microbiology
Vol. 105, H. 2 (2005), S. 123-129
Band:: 105
Heft:: 2
Datum der Veröffentlichung:: 2005
DOI:: 10.1016/j.vetmic.2004.10.010
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: article; assessment; Berlin; diarrhoea; DNA; FOALS; G3; genomic; Genotype; Germany; GROUP-A ROTAVIRUSES; horse; Japan; molecular characterisation; PATTERN; PATTERNS; PCR; POLYMERASE CHAIN-REACTION; probe; reference; REGION; Risk; Risk Assessment; RISK-ASSESSMENT; rotavirus; SAMPLES; SCIENCE; serotype; TIME; TIMES; typing; VP4; Zoonoses
DDC-Sachgruppe der DNB:: 600 Technik
Link URL:: http://www.sciencedirect.com/science/article/pii/S0378113504003827
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014), Fachgruppe 42 - Lebensmittelhygiene und Sicherheitskonzepte
Einrichtung:: Friedrich-Loeffler-Institut, Institut für Bakterielle Infektionen und Zoonosen
Physischer Standort:: SD/2005/106