Validation of the rat limb bud micromass test in the international ECVAM validation study on three in vitro embryotoxicity tests

Spielmann, H.; Genschow, E.; Brown, N .A.; Piersma, A. H.; Verhoef, A.; Spanjersberg, M. Q.; Huuskonen, H.; Paillard, F.; Seiler, A.

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Validation of the rat limb bud micromass test in the international ECVAM validation study on three in vitro embryotoxicity tests

Spielmann, H.; Genschow, E.; Brown, N .A.; Piersma, A. H.; Verhoef, A.; Spanjersberg, M. Q.; Huuskonen, H.; Paillard, F.; Seiler, A.

A detailed report is presented on the performance of the rat limb bud micromass (MM) test in a European Centre for the Evaluation of Alternative Methods (ECVAM)-sponsored formal validation study on three in vitro tests for embryotoxicity. Twenty coded test chemicals, classified as non-embryotoxic, weakly embryotoxic or strongly embryotoxic on the basis of their in vivo effects on animals and/or humans, were tested in four laboratories. The outcome showed that the MM test is an experimentally validated test, which holds promise for use for identifying strongly embryotoxic chemicals, but which needs to be improved before it can be recommended for use for regulatory purposes

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Erschienen in:: Alternatives to laboratory animals : ATLA
Vol. 32, H. 3 (2004), S. 245-274
Band:: 32
Heft:: 3
Datum der Veröffentlichung:: 2004
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: alternative; alternative methods; animal; Animal Testing Alternatives; ANIMAL-EXPERIMENTS; Animals; article; assessment; Berlin; BfR; chemical; chemicals; classification; Comparative Study; Documentation; drug effects; ECVAM; effect; EFFECTS; Embryo; embryology; embryotoxicity; EMBRYOTOXICITY TEST; Europe; evaluation; Extremities; Germany; Human; Humans; In Vitro; in vitro test; in vivo; IN-VITRO; IN-VIVO; international; International Cooperation; Laboratories; Limb Bud; method; methods; PERFORMANCE; Pharmaceutical Preparations; Quality Control; Rat; rats; Reproducibility of Results; Research Support,Non-U.S.Gov't; Risk; Risk Assessment; RISK-ASSESSMENT; studies; study; TESTS; Toxicity Tests; use for regulatory purposes; validation; validation study; VITRO; VITRO EMBRYOTOXICITY TESTS; VIVO; ZEBET
DDC-Sachgruppe der DNB:: 600 Technik
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 3 - Wissenschaftliche Querschnittsaufgaben (bis Juni 2014)