Typing of Salmonella by DNA-microarrays

Malorny, B.; Guerra, B.; Zeltz, P.; Rissler, K.; Helmuth, R.

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Veröffentlicht

Typing of Salmonella by DNA-microarrays

Malorny, B.; Guerra, B.; Zeltz, P.; Rissler, K.; Helmuth, R.

Microarrays (DNA-Chips) are miniaturized carriers on which many nucleic acid molecule probes such as oligonucleotides or PCR products are immobilized in a high density, and compactness. Homologue DNA hybridises with the immobilized complementary nucleic acid probes. This study gives after a short general introduction in the principle of DNA-microarrays an overview about published data on the field of typing of Salmonella by microarrays. An onset of a DNA-microarray developed by the National Reference Laboratory for Salmonella (NRL-Salm) will be introduced. By this new technique, it is possible to answer epidemiological questions as well as to find genes involved in certain biochemical processes, such as pathogenicity or resistance of salmonellae

Einordnung

Erschienen in:: Berliner und Münchener tierärztliche Wochenschrift
Vol. 116, H. 11-12 (2003), S. 482-486
Band:: 116
Heft:: 11-12
Datum der Veröffentlichung:: 2003
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: ACID; Animals; article; Berlin; classification; diagnosis; DNA; DNA MICROARRAY; DNA Probes; Drug Resistance,Microbial; English Abstract; Epidemiology,Molecular; gene; GENES; genetics; Genomic Library; Human; isolation & purification; Laboratories; microarray; microbiology; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; pathogenicity; PCR; probe; product; products; reference; Reference Laboratory; resistance; review; Risikobewertung; SALMONELLA; Salmonella Food Poisoning; Sequence Homology,Nucleic Acid; study; technique; typing
DDC-Sachgruppe der DNB:: 600 Technik
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)