Species identification and detection of vancomycin resistance genes in enterococci of animal origin by multiplex PCR

Mac, K.; Wichmann-Schauer, H.; Peters, J.; Ellerbroek, L.

doi:10.1016/S0168-1605(03)00192-2

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Veröffentlicht

Species identification and detection of vancomycin resistance genes in enterococci of animal origin by multiplex PCR

Mac, K.; Wichmann-Schauer, H.; Peters, J.; Ellerbroek, L.

A multiplex PCR assay for the differentiation of species and detection of vancomycin (van) resistance genes in enterococci was established. Three hundred sixty-seven enterococcal strains isolated from food were selected from a sample collection and were examined with regards to the existence of four vancomycin resistance genes (vanA, vanB, vanC1 and vanC2) and to species differentiation (E. faecalis, E. faecium, E. casseliflavus and E. gallinarum) by multiplex PCR. Apart from unambiguous results for the majority of strains, the E. faecium specific primers used here, showed weak points in the specificity and sensitivity, respectively. Despite these rare instances, we succeeded in optimising the multiplex PCR assay by variation of annealing temperature and primer combination. The assay presented here, with its optimised parameters, is therefore suitable for routine use and because of time and material saving, it is an alternative and rapid method in comparison to conventional tests

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Einordnung

Erschienen in:: International journal of food microbiology
Vol. 88, H. 2-3 (2003), S. 305-309
Band:: 88
Heft:: 2-3
Datum der Veröffentlichung:: 2003
DOI:: 10.1016/S0168-1605(03)00192-2
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: alternative; analysis; animal; Animals; article; ASSAY; Base Sequence; Berlin; Cattle; Chickens; classification; COLLECTION; comparison; consumer; detection; DIFFERENTIATION; DNA; DNA,Bacterial; drug effects; Enterococci; Enterococcus; Enterococcus faecalis; Enterococcus faecium; FAECIUM; Food; Food Microbiology; gene; GENES; genetics; Germany; HEALTH; IDENTIFICATION; method; methods; MULTIPLEX PCR; multiplex-PCR; ORIGIN; PARAMETERS; PCR; PCR assay; Polymerase Chain Reaction; PROTECTION; RAPID METHOD; resistance; resistance genes; SENSITIVITY; Sensitivity and Specificity; species; Species Specificity; SPECIFICITY; STRAIN; STRAINS; Swine; Temperature; TESTS; TIME; VANA; VANCOMYCIN; Vancomycin Resistance; variation; veterinary; Veterinary Medicine
DDC-Sachgruppe der DNB:: 600 Technik
Link URL:: http://www.sciencedirect.com/science/article/pii/S0168160503001922
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)