Eine Molekularbiologische Methodenkaskade zur Detektion, spezifischer Isolierung und Charakterisierung Shigatoxin-produzierender Escherichaia coli (STEC) in Lebensmitteln

Gallien, P.

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Veröffentlicht

Eine Molekularbiologische Methodenkaskade zur Detektion, spezifischer Isolierung und Charakterisierung Shigatoxin-produzierender Escherichaia coli (STEC) in Lebensmitteln

Shigatoxin-producing E. coli (STEC) and their subgroup, the enterohaemorrhagic E. coli (EHEC) are known since about 25 years. Only EHEC can cause diseases in humans. A molecularbiological method cascade is described in this article. It contains the following steps: preenrichment, enrichment, preparation of samples (DNA extraction), screening PCR, specific STEC isolation by using DNA DNA hybridization, verification of isolates as STEC by using PCR, characterization of STEC isolates. Pulsed field gel electrophoresis and DNA sequencing are additional methods for the detection of clonal correlations. They should be performed in special labs, only. Furthermore, some information about an interlaboratory ring trial within Germany and some conclusions are given

Einordnung

Erschienen in:: Berliner und Münchener tierärztliche Wochenschrift
Vol. 116, H. 11-12 (2003), S. 491-495
Band:: 116
Heft:: 11-12
Datum der Veröffentlichung:: 2003
Sprache:: Deutsch
Ressourcentyp:: Text
Schlagwörter:: analysis; Animals; article; Base Sequence; BfR; biosynthesis; chemistry; COLI; Colony Count,Microbial; detection; diagnosis; DISEASE; DISEASES; DNA; DNA Probes; DNA,Bacterial; Electrophoresis; Electrophoresis,Gel,Pulsed-Field; English Abstract; ENRICHMENT; Epidemiologic Studies; Escherichia coli; Escherichia coli Infections; Escherichia coli O157; ESCHERICHIA-COLI; EXTRACTION; Food Contamination; Food Microbiology; gel; gel electrophoresis; GEL-ELECTROPHORESIS; genetics; Germany; Human; Humans; HYBRIDIZATION; INFORMATION; isolation & purification; metabolism; method; methods; microbiology; PCR; Polymerase Chain Reaction; probe; ring trial; Risikobewertung; SAMPLES; Sequencing; Shiga toxin; SHIGA TOXINS; STEC; TOXIN; Toxins
DDC-Sachgruppe der DNB:: 600 Technik
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Abteilung 4 - Biologische Sicherheit (bis Juni 2014)