Detection of tissues of the central nervous system (CNS) as specified risk material (SRM) in meat products by means of gas chromatography-mass spectrometry (GC-MS)

Biedermann, W.; Lucker, E.; Hensel, A.

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Detection of tissues of the central nervous system (CNS) as specified risk material (SRM) in meat products by means of gas chromatography-mass spectrometry (GC-MS)

Determination of specified risk material (SRM) in processed meat products was performed by quantification of brain specific fatty acids using gas chromatography-mass spectrometry (GC-MS). Results from SMP (internal standardised meat products) based analyses showed that absolute concentrations of CNS are correlated (r2 = > 0.97) with the contents of the CNS typical fatty acids docosahexaenoic acid (C 22:6), nervonic acid (C 24:1), lignoceric acid (C 24) and cerebronic acid (C 24oh). GC-MS detection limits were measured at 0.01% CNS. The cut off value was calculated at 0.39% (w/w) CNS in SMP. In a controlled blindfold experiment we were able to identify correctly all positive and negative SMP samples, respectively. Our results indicate that GC-MS based SRM detection may serve as a reference method for immunochemical and immunohistochemical determination of SRM in processed meat products

Einordnung

Erschienen in:: Berliner und Münchener tierärztliche Wochenschrift
Vol. 115, H. 3-4 (2002), S. 131-133
Band:: 115
Heft:: 3-4
Datum der Veröffentlichung:: 2002
Sprache:: Englisch
Ressourcentyp:: Text
Schlagwörter:: ACID; analysis; Animals; article; BRAIN; Brain Chemistry; Cattle; Central Nervous System; CENTRAL-NERVOUS-SYSTEM; cerebronic acid; chemistry; CHROMATOGRAPHY-MASS SPECTROMETRY; CNS; concentration; Consumer Product Safety; detection; Determination; diagnosis; Encephalopathy,Bovine Spongiform; fatty acids; Food Analysis; Food Contamination; Gas Chromatography-Mass Spectrometry; GC-MS; Human; Mass Fragmentography; Meat; Meat Products; MEAT-PRODUCTS; method; methods; nervonic acid; Nervous System; prevention & control; product; products; QUANTIFICATION; reference; Reference Values; Risk; SAMPLES; SPECTROMETRY; SRM; SYSTEM; tissue; tissues; transmission; virology
DDC-Sachgruppe der DNB:: 630 Landwirtschaft, Veterinärmedizin
Einrichtung:: Bundesinstitut für Risikobewertung, Bundesinstitut für Risikobewertung (bis Juni 2014), Leitung